Which post-PCR activity is important to perform?

In post-PCR activities, amplification, probing, and high stringency wash are crucial for ensuring the accuracy and reliability of the results obtained.

Amplification refers to the process of increasing the concentration of specific DNA sequences following PCR, ensuring that the targets are present in sufficient quantity for further analysis. Probing involves the use of specific probes that can bind to the amplified DNA targets; this step is vital for identifying and quantifying the DNA of interest through hybridization techniques. The high stringency wash is critical for enhancing specificity, as it ensures that only well-matched probes remain bound to their targets, while non-specific bindings are washed away. This combination is essential for minimizing background noise and improving the overall quality of results.

Considering the options presented, this particular combination stands out as the most comprehensive and effective for post-PCR procedures.