Certified Histocompatibility Specialist Practice Test

Question: 1 / 400

What is the appropriate dilution volume required to achieve a working concentration of 40ng/ul from a 100ul stock solution at 167ug/ml?

418ul

To achieve a working concentration of 40 ng/µL from a stock solution of 167 µg/mL, it is essential to understand the relationship between concentration, volume, and dilution.

First, convert the stock concentration from µg/mL to ng/µL to make it compatible with the desired units. Since \(1 \, \text{µg/mL} = 1 \, \text{ng/µL}\), the stock concentration of 167 µg/mL is equivalent to 167 ng/µL.

Next, use the dilution formula, which can be expressed as follows:

\( C_1V_1 = C_2V_2 \)

Where:

- \( C_1 \): initial concentration (167 ng/µL)

- \( V_1 \): volume of the stock solution needed

- \( C_2 \): target concentration (40 ng/µL)

- \( V_2 \): final total volume of the diluted solution

We need to find \( V_2 \) in order to determine the dilution volume. Rearranged, the equation becomes:

\( V_2 = \frac{C_1V_1}{C_2} \

Get further explanation with Examzify DeepDiveBeta

4180ul

318ul

3180ul

Next Question

Report this question

Download Certified Histocompatibility Specialist Practice Test PDF Study Guide
Subscribe

Get the latest from Examzify

You can unsubscribe at any time. Read our privacy policy