Which DNA typing method is improperly matched with a critical methodological step?

The choice that is identified as improperly matched relates to the Reverse SSO method and its connection to a high stringency wash. In Reverse SSO (Solid-Phase Oligonucleotide), the methodology typically does not require high stringency washes to the extent that other methods do, such as SSOP (Sequence-Specific Oligonucleotide Probes). High stringency washes are crucial in SSOP to ensure that only perfectly matched probes remain bound to the target DNA, thus increasing specificity.

In contrast, Reverse SSO's methodological framework can tolerate a different washing technique, which allows for a broader range of binding conditions. This is because the design of the Reverse SSO method employs a different approach to hybridization and target capture that doesn't necessitate the same level of stringent washing as SSOP. Therefore, the mismatch of Reverse SSO with high stringency wash delineates a significant difference in the procedural requirements between these DNA typing methodologies.