What is the minimum blood volume needed for testing if 2,000 lymphocytes/well are required?

To determine the minimum blood volume needed for testing if 2,000 lymphocytes per well are required, it is essential to understand the context of lymphocyte extraction from blood.

Given that lymphocytes constitute about 20% of the total white blood cell (WBC) count, you need to consider the typical concentration of WBCs in human blood, which is around 4,000 to 10,000 cells per microliter (µL). For calculation purposes, let's assume an average WBC count of 7,000 cells/µL. This means that in every milliliter (mL) of blood, there are approximately 7 million WBCs.

If we need to obtain 2,000 lymphocytes, we can calculate the required blood volume as follows:

1. Calculate the number of total WBCs needed based on the lymphocyte proportion:

   • Since lymphocytes are about 20% of WBCs, to get 2,000 lymphocytes, the total number of WBCs required will be:
   • 2,000 lymphocytes ÷ 0.20 = 10,000 total WBCs.

2. Determine the volume of blood required to yield 10