What is the effect of heterophile antibodies in rabbit complement during the AHG-CDC assay?

In the context of the AHG-CDC assay, the presence of heterophile antibodies in rabbit complement plays a significant role in the assay's effectiveness. Heterophile antibodies are antibodies that can react with various species but are not specific to the target antigens being tested. Their effect in the rabbit complement system is notable because they can enhance the effectiveness of the complement by promoting the hemolytic activity in the assay. This enhancement occurs as heterophile antibodies can cross-link the complement components more effectively, leading to a more robust formation of the immune complex that activates the complement pathway, thus increasing the lysis of target cells.

The functioning of the AHG-CDC assay relies on the complement system effectively lysing target cells that are sensitized with antibodies. When the heterophile antibodies are present, they facilitate this process, ultimately leading to improved sensitivity and specificity in detecting antigens. This enhancement is essential for the accurate interpretation of results in histocompatibility testing, as it can significantly influence outcomes and the assessment of compatibility in transplant scenarios.