What is required when reconstituting lyophilized antibodies for flow cytometry according to ASHI standards (2005)?

When reconstituting lyophilized antibodies for flow cytometry, one essential requirement according to the ASHI standards is the removal of microaggregates. This is critical because microaggregates can interfere with the flow cytometry results by affecting the consistency and accuracy of the analysis. These aggregates may form during the lyophilization process or during the reconstitution, and their presence can lead to clumping of particles that would distort the flow cytometry readings. Ensuring that the antibodies are free of these microaggregates helps maintain the integrity of the sample and provides reliable and reproducible results.

The quality of the antibodies is paramount for accurate flow cytometry applications, and adhering to this standard aids in achieving optimal performance during experiments. Properly reconstituted antibodies contribute to the overall reliability of the assays conducted, reinforcing the importance of this particular step in the process.