If A+Ax is changed to 5000 CPM, what would be the consequence for response calculations against Dad?

The response calculations against Dad, particularly in the context of histocompatibility and immunology, often involve a scrutiny of two essential parameters: the Stimulation Index (SI) and the Relative Response Index (RRI). When adjusting the baseline for A+Ax to 5000 CPM—a relatively high count per minute—it's crucial to understand how this would reflect in the calculations.

When the CPM is increased substantially, it typically indicates a higher level of background or baseline stimulation, which can influence the SI. The SI is determined by comparing the responses of the experimental group (in this case, the reaction to Dad’s antigen) against a baseline control. If the CPM is high, it may result in a dilution of the specific response being observed. Consequently, this can lead to a reduction in the SI.

Similarly, the RRI, which contrasts the response of test samples to a control or reference, may also show a decrease in sensitivity to differences in antigen recognition when faced with a higher CPM background. The increase in CPM likely saturates the response, impairing the ability to distinguish meaningful differences between test responses and the controls.

Therefore, setting A+Ax to 5000 CPM would decrease both the Stimulation Index and the Relative Response Index,