Ficoll-Hypaque is used to separate lymphocytes primarily based on which property?

Ficoll-Hypaque separates lymphocytes primarily based on density. This density gradient centrifugation method utilizes a Ficoll solution that has a specific density, allowing cells to be stratified according to their own densities during the centrifugation process. Lymphocytes, being less dense than other cells present in blood, will float to a particular layer, which can then be collected for further analysis or experimentation.

This technique is particularly effective because lymphocytes have a distinct density compared to red blood cells, granulocytes, and other blood components. The other properties listed, such as size, adherence, and color, do not significantly influence the efficacy of Ficoll-Hypaque in the separation of lymphocytes, making density the crucial factor in this context.